Key Words:
Nosocomial infection, bacteria, ethanol, concentration

ABSTRACT
In order to reduce the nosocomial infections among hospitalized patients and visitors a study was conducted through isolation of microbes and analysis of the activity of ethyl alcohol against the isolated organisms. In our study we found that Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae were found predominant. The Gram positive isolation by open plate method and swabbing from various areas showed the isolation of Gram positive pathogens and accounted for 58% of nosocomial infections and Gram negative pathogens accounted for 42%. As a result of this investigation, alcohol activity showed better inhibition against various isolated bacterium, where Gram positive bacteria showed more inhibition than Gram negative bacteria. Its clear that the Gram positive bacteria inhibited at the concentration of 60% of ethanol whereas the Gram negative showed partial inhibition even though at 80%. This study gives an idea about application of alcohol in health care industry, especially in cleaning of walls, tables and other inanimate objects available in the indoor environment.